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SAMPLING PROCEDURES

Sampling procedures for METABOLIC PROFILE TEST;
Metabolic profile tests should be routinely performed three times a year. The tests are carried out in summer, autumn and winter.Tests except of these periods can also be applied to determine the decrease in efficiency performance like fertility and milk yield because of increasing health problems in the future. (Radostitis OM et al., 2004, Quiroz-Rocha GF et al., 2010, Kida K., 2002).
Taking blood samples is one of the important steps for accurate diagnostic results.If a change takes place in ration, the samples should be taken and tested at the end of the two weeks as adaptation period for the new ration.Blood samples should be delivered to the appropriate blood tubes (red capped tube, serum capped tube, green capped tube any of them) for biochemical evaluation 2-3 hours after feeding, if possible, after removed serum in the cold chain without exposure to light.(LeBlanc S. 2010,, Quiroz-Rocha GF ve ark. 2010, Kida K. 2002).
Sample Picking Animals;
First of all the herd must be divided into groups. Generally, it is more than enough to separate four groups as early dry period, late dry period, early lactation and late lactation.
Early dry period group: cows are separated from cows that have been separated for 10 days to dry period and cows that have been born for 30 days.
Late dry period group: It is formed from 3 to 21 day cows to born.
Early lactation group: It is formed from cows between the 3rd and the 30th day of lactation.
Late lactation group: It is formed from cows with 30th day and further of lactation.
Animals to be sampled;
must have a yield average close to the yield average of the group.
They should be healthy.
Records of all the information of the selected animals must be available.
(Radostitis OM et al., 2004, Van Saun RJ, 2007).

Herd Information
In order for the test results to be accurately assessed, it is necessary to have sufficient knowledge of the herd,
Total number of animals in the herd,
All records about the farm,
Shelter conditions,
Applied rationing programs,
Types and quantities of coarse and concentrates feed used in the field,
Analyzes of used feed,
Adequate information should be provided to assess the test results accurately regarding the number of cows in lactation, the total amount of milk produced, and the milk fat ratio.
(Van Saun RJ, 2007).

Individual information about animals in the test group
The age of the animal,
Daily milk production,
Whether or not mastitis,
Number of calving,
Dates of calving,
Consumption of Daily amount of dry matter,
Body weight,
Nutritional status,
This information will help to ensure that the test results are interpreted in the most accurate way, reducing the margin of error (Radostitis OM et al., 2004, Van Saun RJ, 2007).

Sampling Procedures from Coarse Feed;

Sampling Procedures from Silage;

Sampling Procedures from TMR;